Polysulphanilamidoaromatic, polysulphanilamidoaliphatic and related compounds and a process for making them



Patented Oct. 7, 1941 I UNITED I STATES PATENT OFFICE:

POLYSULPHANEAMIDOAROMATIC,

SULP ALIPHAIIC LATED COMPOUNDS AND A PROCESS FOR.

MAKING THEM roLr- AND BE- Elmore H. Northey, Bound Brook, and Martin E.

Hultquist, North Plainfleld, N. .L, assignors, by mesne assignments, to American Cyanamid Company, New York, N. Y., a corporation of Maine No Drawing. Application April 16,1938, Serial No. 202,450

19 Claims.

may be represented by the following general forin which R1 is a para aminoaryl radicaLRz is a residue of an aromatic or aliphatic polyamine, R3 is an aromatic radical, X is hydrogen, alkyl or substituted alkyl and n is a small whole number, are of importance as chemo-therapeutic agents against bacterial and virus infections and many of them may also be used as dyestufl intermediates. The presence of at least one para aminoarylsulphon group is an essential feature of the present invention as most effective therapeutic agents contain at least one of these groups. It will be noted that all of the compounds are sulphonamide derivatives of an aromatic or aliphatic polyamine, the residue of which is given as R2 in the general formula.

The sulphonamide derivatives of the polyamines may be of the most various nature so long as at least one para aminoarylsulphon radical is united to one of the amino groups. The best therapeutic agents are obtained where both the radicals R1 and R3 are aminoaryl radicals and particularly para aminoaryl radicals and these constitute the preferred members of the class covered by the present invention.

The various aryl radicals are preferably unsubstituted except by amino, sulphonamido and sulphonic groups where maximum activity is desired ,but substituted compounds in which the substituents may be alkyl, halogen, nitro, and the like are useful as dyestuif intermediates and are included in the present invention.

The most important compounds of the present invention are those in which the various aryl radicals belong to the benzene, diphenyl and naphthalene series but the invention is in no sense limited to such compounds. On the contrary,-other aryl radicals such as diphenyloxide, anthracene, phen'anthrene and the like may be present.

While the invention is not broadly limited to any particular process, I have found that a very desirable process, particularly for the production of symmetrical compounds, is to react acylaminoarylsulphonchlorides on the desired aromatic or aliphatic diamine and then hydrolyzing the acylamino groups if the unacylated amino compound is desired. In the examples the most common acyl group, namely, the acetyl group, is described. The particular acyl group does not materially affect the process and any of the other common acyl groups may be substituted for the acetyl p.

The invention will be described in detail in conjunction with the following specific examples which illustrate typical products covered in the present invention and methods for making them. The parts are by weight.

EXAMPLE 1 1,2-Bis-sulphanilamidobenzene S OzNH S OaNH 468 parts of freshly prepared N-acetylsulphanilyl chloride are added with eflicient agitation over a period of two hours to 108 parts of fresh ortho phenylenediamine dissolved in 2000 parts of water containing 40 parts of soda ash. 50% sodium hydroxide is added as required to maintain the pH of the mixture at 8 to 9 and the temperature is held at approximately 40 C. Under these conditions the soluble disodium salt of 1,2-bis-(N-acetylsulphanilamido) benzene is formed; at higher or lower pHs, material tends to precipitate out.

A sample of the solution is titrated with N/ 10 nitrite solution. If more than .05 equivalent of free amino nitrogen is present, additional N- acetylsulphanilyl chloride is added until there is no further reduction in free amino nitrogen.

Hydrolysis of the acetyl groups may be effected by adding 200 parts of caustic soda to the solution of disodium 1,2-bis-(N-acetylsulphanilamido) benzene and boiling until there is no further increase in diazotizable amino groups. This will normally take from 1 to 2 hours. alternate procedure of hydrolysis consists in acidifying the solution with hydrochloric acidto a pH of 4 to 5 and crystallizing the intermediate compound obtained which is then filtered andwashed. This intermediate compound may be hydrolyzed by boiling with 250 parts of sodium hydroxide dissolved in 1000 parts of water. This second procedure. eliminates some highly colored imgurities and makes the subsequent purification ea er.

In either case, purification may be effected by acidifying the hydrolysis mixture to a pH of to 6 and filtering. The liquor is discarded and the crystals dissolved in 1000 parts of hot water with an excess of hydrochloric acid, treated with decolorizing carbon and a pinch of sodium hydrosulphite and then neutralized with sodium hydroxide. The sticky precipitate is filtered oi! and recrystallized twice from 1500 cc. of 60% alcohol using decolorizing carbon.

The final product is a white crystalline compound which melts at 187.3 to 188.7 C. Assay by nitrite indicates 100.3%.

The corresponding naphthalene compound can be prepared by replacing the ortho phenylenediamine with an equivalent quantity of a naphthalenediamine, such as 1,4-diamirionaphtha1ene.

EXAMPLE 2 1,3-Bis-sulphhnilamidobenzene NH s 00ml. NHr-OS ohm-O The procedure of Example 1 is followed, replacing the ortho phenylenediamine by an equal amount of meta phenylenediamine. Crystallization of the crude 1,3-bis-sulphanilamidobenzene is best effected from acetone or from an acetonewater mixture. The purified product must be dried at 90 C. under a vacuum to remove water of crystallization.

The white, crystalline compound melts at 220.5 to 221.7 C. and assay by nitrite indicates 100.0%.

EXAMPLE 3 1,4-Bis-sulphanilamidobenzene NHQS OzNHGNHSOrONH:

Exam: 4

1,3,5-Trisulphanilamidobenzene NH S DONE:

NET-GS OINH NHSOQ-NH! 77.5 parts of 1,3,5-triaminobenzene trihydrochloride dissolved in 500 parts of water are neutralized with 50% sodium hydroxide solution and 20 parts of soda ash and 2 parts of sodium hydrosulphite added. 234 parts of N-acetylsulphanilylchloride are then added withvigorous agitation over a period of 2 hours at 35 0., maintaining a pH of 8 to 11 by addition of 50% sodium hydroxide solution as necessary. Additional small amounts of N-acetylsulphanilyl chloride are added until the. test for diazotizable amine reaches a minimum value. The reaction mixture is then acidified to a pH of 3 to 4 and the heavy precipitate filtered oil and washed with water.

Hydrolysis oi the acetyl groups is effected by; boiling the precipitate with a mixture of 120', parts of sodium hydroxide and 500 parts oi water{ for two hours. The dark brown solution obtained is diluted to 2000 parts, treated first with decolorizing carbon and then with about 1 part of sodium hydrosulphite, and then acidified with hydrochloric acid to a pH of 5 to 6. The heavy gray precipitate is dissolved and reprecipitated twice, then dissolved in a mixture of 1500 parts 01 acetone and 1000 parts of hot water and treated three times with decolorizing carbon.

Crystallization of the product is brought about by evaporating oi! about 800 parts of acetone under a vacuum. The crude crystals obtained are filtered off, washed with acetone and recrystallized from 60% acetone.

Nitrite titration gives an equivalent weight of 197 as against 196.2 of theory and the 1,3,5-trisulphanilamidobenzene decomposes at 335 to 340 C.

Exmu: 5

1,2-Bis-sulphanilamidoethane 234 parts of freshly prepared N-acetylsulphanilylchloride are added over a period of 1 hour to 30 parts of ethylenediamine dissolved in 1500 parts of water at a temperature of 40 to 45 C. and a pH of 8 to 10 maintained by addition of 50% sodium hydroxide solution as necessary. The mixture is stirred for 1 hour, aiter which 120 parts of sodium hydroxide are added and the mixture boiled for two hours. The hydrolysis mixture is neutralized to a pH of 5 to 6 with hydrochloric acid and the crystalline precipitate filtered off.

Purification is best effected by dissolving the crude 1,2-bis-sulphanilamidoethane in 1000 parts of water with a minimum amount of sodium hydroxide, treating with decolorizing carbon and reprecipitating with acid. The precipitate is then dissolved in 1000 parts of water containing hydrochloric acid, treated with decolorizing carbon, neutralized with sodium hydroxide and washed free of salt with distilled water. The white crystalline compound, almost insoluble in water but readily soluble in strong acids or bases, melts at 228 to 230 C, with slow decomposition. Assay by nitrite indicates EXAMPLE 6 N ,N--Bis- (Z-sulphanilamidoethyl) sulphamlamide NHr-OSmNwmCHr-NHSm-ONEM sulphanilamide trihydrochloride is recrystallized ,from 500 parts of water using decolorizing carbon.

Nitrite titration gives an equivalent weight 227.3 as against the theory or 228 and the compound melts at 241.5" to 244 C.

Exam 7 Sodium 3,4-bis-sulphanilamidobenzenesulphmiatc I poem SOrNa The procedure of Example 1 is followed starting with 210 parts of freshly prepared sodium 1,2-diaminobenzene-4-sulphonate instead of the ortho phenylenediamine and only half the quantity or water is needed.

Purification oi' the crude product is efiected by dissolving at a pH of 5 to 6 with sodium hydroxide, treating first with decolorizing carbon, then with a little sodium hydrosulphite and then slowly acidifying to a pH 01' 2 to 3. Several recrystallizations are necessary to remove the highly colored impurities present. The sodium salt or the compound is finally obtained on cooling the clarified solution at a pH of 5 to 6. The white crystalline product is dried at 100C after which nitrite titration gives an equivalent weight of 259 as against a theory of 260.2. The compound gradually melts to a viscous mass above 200 C.

Exam 8 Sodium 2,4-bis-sulphanilamidobenzenesulphonate SO4Na rim-Os olNaQ HSOONHI The procedure of Example 7 is followed starting with sodium 2,4-diaminobenzenesulphonate instead of sodium 1,2-diaminobenzene-4-sulphonate. The crude product is recrystallized by dissolving at a pH of 9 to 10 and reprecipitating at a pH of 5 to 6. The monosodium salt is spar-' ingly soluble in water but very soluble in acids or bases.

Sodium nitrite titration gives an equivalent weight of 260.4 as against a theory of 260.2.

EXAMPLE 9 Sodium 2,5-bis-su1phanilamidobenzenesulphonate 8 ON a Exmru: 10

Sodium, 2,4-bis-sulphanilamidotoluene-5- sulphonate 8 OaNa so NH:

The procedure oi Example 7 is followed starting with 224 parts of sodium 2,4-diaminotoluene- 50% alcohol using decolorizing carbon.

fi-sulphonate instead of sodium 1,2-diaminobenzene-4-sulphonate. The product decomposes above 300 C.,and a sodium nitrite titration gives an equivalent weight oi 266 as against the theory of 267.2.

Exmu 11 Disodium 2.4-bis-sulphanildmidobenzene-L5- dlsulphonate NHQSMNHOS OzNa NH s 001m,

The procedure oi! Example 'I is followed starting with 268 parts oi! 2,4-diaminobenzene-L5-disulphonic acid. When the hydrolysis mixture is acidified to a pH of 3 to 4 sulphanilic acid precipitates first. The filtrate is neutralized to a pH of 7 to 8, concentrated, cooled and the crystalline precipitate separated and recrystallized from The white crystalline compound gives an equivalent weight of 305 by sodium nitrite titration as against 311.2 of theory for the disodium salt.

Exanru: 12

Sodium 2,4-bis-(N-sulphanilylsulphanilamido) benzenesulphonate NIL-OS mNH-O-s 0:-NH

' NHOS olNnOs 0.1m

OsNa

lution is added from time to time to maintain a-pH of 8 to 10. Additional small portions of N-acetylsulphanilylchlcride are added until a minimum value is reached when testing for diazotizable amine. The reaction mixture is then acidified to a pH of 1 to 2 and the sticky precipitate separated and hydrolized by boiling with 200 parts of sodium hydroxide and 1500 parts of water until there was no further increase in diazotizable amino nitrogen (approximately 2 hours). A tally-like mass of crude product, obtained when the solution is cooled and acidified to a pH of 1.5 to' 2.5, is separated, dissolved in 3000 parts of boiling water with the addition of a little sodium hydroxide solution to bring the pH to about 3.5, treated with decolorizing carbon and the filtrate treated with about 1 part of sodium hydrosulphite. The product again separates as an amorphous mass when re-acidifled and is dissolved in 1500 parts of hot water with sufficient sodium hydroxide to bring it to a pH of 5 to 6. It is again treated with decolorizing carbon and set aside to crystallize overnight. When the crystals are filtered oil, washed, and dried in a vacuum at C., a sodium nitrite titration shows an equivalent weight of 428 as against the theory of 431.

Examru: 13 lent weight of 200 as against a theory of 200.2. 1,2-Bis-(N -sulphanilylsulpltanilamido) ethane The compound melts at 184'2 to 186's Nm-O-SOMHOB OzNH-CHaCHr-NH s m-O-nns 0,

The procedure of Example 12 is followed us- EXAMPLE 16 trite titration gives an equivalent weight 01 33a 15 P'mate as against 340.2 for theory. The product melts indefinitely from 120" to 160 0., forming a clear, NHOSONH O glass-like brittle solid on cooling. O Na EXAMPLE 14 OiNa 1-N -(p-toluenesulphonyl)-sulphanilamido-4- 344 parts of benzidine-2,2'-disulphonic acid is (p-toluenesulphanilamide) benzene dissolved in 3000 parts of water with 80 parts of .400 parts of para toluenesulphonylchloride are caustic soda and 75 parts of soda ash. A paste added with agitation over a period of a half hour containing 468 parts of acetylsulphanilyl chloride at 50 to 60 C. to a suspension of 130 parts of is gradually added with agitation while main- 4-aminosulphanilanilide in 2500 parts of water taining the pH from 8 to 10 by addition of 50% and parts of soda ash, the pH being kept at 9 caustic soda solution as necessary. The temto 11 by the addition of 50% sodium hydroxide perature is held from 40 to 50 C. by th ad solution as necessary. The temperature is then tion of ice. When reaction is complete, as indiraised to 80 to 90 C. and held there until the cated by a nitrite titration, the reaction mixture test for diazotizable amine disappears and the a is acidified and a clear jelly is formed. The mixexcess para toluenesulphonylchloride is hydroture can be precipitated in a form in which it is lyzed. The solution is treated first with decolorreadily flltera e y ding concentrated hy r izing carbon, then with a small amount of sodium chloric acid to the solution at 60 to 70 C. until hydrosulphite and then acidified with hydroa pH of 2 to 3 is obtained and then coolingvery chloric acid. The dark amorphous mass obslowly, Preferably ight. The filter cake is tained is redissolved in 2500 parts of alcohol, hydrolyzed by boiling for 1 hours with 300 treated with decolorizing carbon four times and parts of concentrated hydrochloric acid and 200 then poured into 10,000 parts of cold water. A parts of water. The slurry is cooled and filtered, white amorphous precipitate is obtained which is the filter cake dissolved in 500 parts of boiling readily filterable and after drying at 50 C. the water with addition of caustic soda until neutral, material melts indefinitely from 100 to 150 C., then treated with decolorizing carbon, cooled and forming a clear, glass-like brittle solid on cooling. the disodiumsalt permitted to crystallize out.

The crystals are dried at 85 C.

, EXAMPLE 15 1,3-Bis-sulphanilamidopropanol-2 EXAMPLE 17 NHrOSOaNH omoHoHom-nnsm-O- 4,4-DisuZphanilamid0stilbene-2,2'-disulphonic 468 parts of N-acetylsulphanilyl chloride are acid NHrO-S omnOoH=cnONn s Oz-O-NH:

added with eflicient agitation over a period of an 184 parts of 4,4-diaminostilbene-2,2'-disulhour at 40 to 45 C. to 86 parts of 1,3-diaminophonic acid are dissolved in 1000 parts of water propanol dissolved in 800 parts of water, the pH with 80 parts of caustic soda and 30 parts of soda being maintained at 8 to 10 by the addition of ash. A paste containing 281 parts of acetylsulsodium hydroxide as necessary. The crude rephonilyl chloride is gradually added with agitaaction product separates to form a slurry. This tion, keeping the reaction mixture at a pH of 8 is dissolved in excess sodium hydroxide, the soluto 10 by addition of 50% caustic soda solution as tion clarified and acidified with hydrochloric necessary. The temperature is held at 40 to 50 acid. The crude diacetyl compound is filtered 011 5 C. by the addition of ice.

and boiled with 200 parts of sodium hydroxide The solution is clarified and slowly acidified and 800 parts of water for 2 hours. The hydrolw h hy r h acid to pr ipi ,4'- aceysis mixture is diluted to 3000 parts and acidified y ulp all lam dostilbene-2,2-disulp d with hydrochloric acid. The resulting white in ys all fO m. The acidity should be such precipitate is filtered off, dissolved in 1500 parts that Congo just turns red. The mixture is filof water and 250 parts of concentrated hydrotered and the filter cake washed to remove any chloric acid, treated with decolorizing carbon and colored impurities. The cake is then boiled for then neutralized with sodium hydroxide. The 4 hours with 250 parts of concentrated hydroproduct is finally recrystallized from 50% alcochloric acid. After the hydrolysis has been comhol. Sodium nitrite titration gives an equiva- 7 pleted, the slurry is filtered and the precipitate thoroughly washed with water. The freev acid is very insoluble in water and is recrystallized by forming the sodium salt, dissolving in water and reprecipitating with acid. The material is sensitive to light and oxidation.

What we claim is:

1'. Sulphonamido compounds having the following general formula:

' in which R1 is a para aminoaryl radical linked to the SO: group through a nuclear carbon atom, R2 is a polyvalent radical included in the group consisting of polyvalent aryl and open chain saturated aliphatic radicals, R3 is an aryl radical, X is a radical included in the group consisting of hydrogen and radicals of the paraiiin series, and n is a small whole number.

2. Sulphonamido compounds. having the following general formula:

in which R1 is a para aminoaryl radical linked to the S02 group through a nuclear carbon atom, R2 is a polyvalent aryl radical, R: is an aryl radical, X is a radical included in the group consisting of hydrogen and radicals of the paraflin series, and n is a small whole number.

'4. Sulphonamido compounds having the following general formula:

in which R1 is a para aminoaryl radical linked to the S: group through a nuclear carbon atom, R2 is a polyvalent saturated open chain aliphatic radical, R: is an aryl radical, X is a radical included in the group consisting of hydrogen and radicals of the paramn series, and n is a small whole number.

5. Sulphonamido compounds having the following general formula:

in which R1 is a para aminoaryl radical linked to the S02 group through a nuclear carbon atom, R2 is a polyvalent radical of the benzene series, R3 is an aryl radical, X is a radical included in the group consisting of hydrogen and radicals of the paraffin series, and n is a small whole number.

6. Sulphonamido compounds having the following general formula:

in which R1 and Rs are para aminoaryl radicals linked to the S02 groups through nuclear carbon atoms, R: is a polyvalent radical of the benzene series, X is a radicalincluded in the group consisting of hydrogen and radicals of the paraflin series, and n is a small whole number.

7. Sulphonamido compounds having the following general formula:

in which R1 and R3 are para aminoaryl radicals linked to the S02 groups through nuclear carbon atoms, R2 is a meta phenylene radical, and X is a radical included in the group consisting of hydrogen and radicals of the paraflin series.

8. Sulphonamido compounds having the following general formula:

in which R1 and R3 are para aminoaryl radicals linked to the S02 groups through nuclear carbon atoms, R2 is a para phenylene radical, and X is a radical included in the group consisting of hydrogen and radicals of the parafdn series.

9. Sulphonamido compounds having the following general formula:

in which R1 and R: are para aminoaryl radicals linked to the S02 groups through nuclear carbon atoms, R: is a sulphonated phenylene radical, and X is a radical included in the group consisting of hydrogen and radicals of the parafiin series.

10. Sulphonamido compounds having the following general formula:

SOzNH-R-NHS O NH:

in which R is a phenylene radical.

ll. Sulphonamido compounds havingthe following general formula:

OaNH-R-NHSO NH;

in which R is a meta phenylene radical.

12. Sulphonamido compounds having the following general formula:

in which R is a para phenylene radical.

13. The sulphonamido compound having the following formula:

where-X is a radical included in the group consisting of hydrogen and alkali metals.

14. The sulphonamido compound having the following formula:

NHQQINHQ-SOINH Nm-O omnOsoam solx where X is a radical included in the group consisting of hydrogen and alkali metals.

15. A method of preparing sulphonamide compounds having the following general formula:

in which R1 is a para aminoaryl radical linked to the SO: group through a nuclear carbon atom, R2 is a polyvalent radical included in the group consisting of polyvalent aryl and open chain saturated aliphatic radicals, R3 is an aryl radical, X is a radical included in the group consisting of hydrogen and radicals of the paraffin series, and-n is a small whole number, which comprises reacting N-acylarylsulphonyl halides on a polyamine included in the group consisting of aryl polyin which R; and Rs are para aminoaryl radicals linked to the SO: groups through nuclear carbon atoms, R: is a polyvalent aryl radical, X is a radical included in the group consisting of hydrogen and radicals of the paraffin series, and n is a pounds having the following general formula:

small whole number, which comprises reacting para aoylaminoarylsulphonyl halide on a diamine included in the group consisting of aryl diamines and aliphatic diamines, the acyl group being the acidic residue of an organic carboxylic acid, the amount of the para acylaminoarylsulphonyl halide being at least twice the stoichiometric equivalent of the diamine.

18. A method of preparing sulphonamide comin which R1 and Rt: are para aminoaryl radicals linked to the S02 groups through nuclear carbon atoms, R: is a polyvalent radical included in the group consisting of arylene and alkylene radicals, and X is a radical included in the group consisting 0! hydrogen and radicals of the parafiin series,

which comprises reacting a para acylamlnoarylsulphonyl halide with a diamine included in the group consisting of aryl and aliphatic diamines, the acyl group being the acidic residue of an organic carboxylic acid, the amount of the para acylaminoarylsulphonyl halide being at least twice the stoichiometric equivalent of the diamine. I

19. A method of preparing sulphonamide compounds having the !ollowing general formula:

N HOS OaNH-R-N HSOPONH:

wherein R is a phenylene radical, which comprises reacting an N-acetylaminobenzenesulphonyl halide with a phenylene diamine, the

amount of the N-acetylaminobenzenesulphonyl halide being at least twice the stoichiometric 7 equivalent of the diamine.

ELMORE H. NORTHEY. MARTIN E. HULTQUIST. 

